WormBase Tree Display for Strain: WBStrain00054870
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| WBStrain00054870 | Genotype | unc-119(ed3) III; bcSi69 IV; bcEx1368. | ||
|---|---|---|---|---|
| Public_name | MD4572 | |||
| Contains | Gene | WBGene00006843 | ||
| Variation | WBVar00145093 | |||
| Transgene | WBTransgene00033493 | |||
| Properties | Outcrossed | x0 | ||
| CGC_received | 11 Jul 2023 00:00:00 | |||
| Location | CGC | |||
| Made_by | WBPerson7012 | |||
| Remark | bcSi69 [hsp-16.41p::dCas9::SV40-NLS::HA::GS-linker::SV40-NLS::GFP::GFP::SV40-NLS::mai-2 3UTR + Cbr-unc-119(+)] IV. bcEx1368 [U6p::rrn-1(sgRNAs1-4 targeting the rrn-1 locus) + rol-6(su1006)]. Maintain at 15C. Pick Rollers to maintain. After heat shock, distinct spots of GFP fluorescence are visible in the nucleus. bcEx1368 array contains a PCR product of 4 different guide RNAs targeting rrn-1 (around 100 repeats of rrn-1 on LG I). Reference: Memar N, et al. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462. | Inferred_automatically | From CGC strain data | |
| Species | Caenorhabditis elegans |
