WormBase Tree Display for Transgene: WBTransgene00000282
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| WBTransgene00000282 | Public_name | cgc3632Is1 | |
|---|---|---|---|
| Summary | [lin-36::gfp] | ||
| Construction | Construct | WBCnstr00000282 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | Gamma_irradiation | ||
| Construction_summary | lin-36::GFP was injected in 50 ug/ml together with pRF4 80ug/ml as marker. | ||
| A lin-36::GFP reporter was constructed by ligating a 4.4 kb SalI SacI fragment from pJHT16 to a linker fragment and SalI XmaI double-digested pPD95.79, a vector containing a green fluorescent protein (GFP) coding region with a C. elegans unc-54 3 end and a fluorescence enhancing S65T mutation. The translational fusion contains 1 kb of sequence 5' to the ATG start site, the entire lin-36 open reading frame, all lin-36 introns and the GFP gene fused in-frame to the last codon of lin-36. | |||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr1154 | |
| Reference | WBPaper00003632 | ||
| Species | Caenorhabditis elegans |
