WormBase Tree Display for Transgene: WBTransgene00000302
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WBTransgene00000302 | Public_name | cgc5278Is1 | |||||||
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Summary | [pry-1::gfp] | ||||||||
Construction | Construct | WBCnstr00000302 | |||||||
Integration_method | Gamma_irradiation | ||||||||
Construction_summary | translational fusion with 3.6 kb of upstream sequence of pry-1. | ||||||||
Genetic_information | Integrated | ||||||||
Phenotype | WBPhenotype:0000469 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Overexpression of PRY-1 or a PRY-1::GFP fusion protein resulted in a highly penetrant QL.d migration defect (Fig. 3A; data not shown)... In wild-type and pry-1(mu38) animals, the QL.pa daughter cells were found at their normal position (near V5.a), but in animals that overexpress PRY-1, the QL.pa daughter cells localized far into the anterior... We also observed that the QL and QR daughter cells migrated further into the anterior than the QR.d in wildtype animals (Fig. 3A), a phenotype that is also observed in lin-17/Fz and bar-1 mutants (Whangbo et al. 2000)." | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Caused_by_gene | WBGene00004202 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0004056 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0004086 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0004993 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0004054 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001278 | Paper_evidence | WBPaper00005278 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We found that 40/40 wild-type animals expressed the mab-5::lacZ reporter in the QL daughter cells (Fig. 3B). In animals that overexpress PRY-1, no mab-5::lacZ expression was observed in the QL.d (n = 40), indicating that PRY-1 overexpression inhibits mab-5 expression." | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Caused_by_gene | WBGene00004202 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0004056 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | muIs2 [mab-5::lacZ] | Paper_evidence | WBPaper00005278 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_not_observed | WBPhenotype:0000232 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "The localization of other migratory neurons, such as the HSN and CAN neurons, was not affected (n = 26) by PRY-1 overexpression." | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Caused_by_gene | WBGene00004202 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006827 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0000470 | Paper_evidence | WBPaper00005278 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "The localization of other migratory neurons, such as the HSN and CAN neurons, was not affected (n = 26) by PRY-1 overexpression." | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Caused_by_gene | WBGene00004202 | Paper_evidence | WBPaper00005278 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006830 | PATO:0000460 | Paper_evidence | WBPaper00005278 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Used_for | Expr_pattern | Expr1896 | |||||||
Interactor | WBInteraction000538501 | ||||||||
Reference | WBPaper00005278 | ||||||||
Species | Caenorhabditis elegans |