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WormBase Tree Display for Transgene: WBTransgene00000302

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Name Class

WBTransgene00000302Public_namecgc5278Is1
Summary[pry-1::gfp]
ConstructionConstructWBCnstr00000302
Integration_methodGamma_irradiation
Construction_summarytranslational fusion with 3.6 kb of upstream sequence of pry-1.
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000469Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Remark"Overexpression of PRY-1 or a PRY-1::GFP fusion protein resulted in a highly penetrant QL.d migration defect (Fig. 3A; data not shown)... In wild-type and pry-1(mu38) animals, the QL.pa daughter cells were found at their normal position (near V5.a), but in animals that overexpress PRY-1, the QL.pa daughter cells localized far into the anterior... We also observed that the QL and QR daughter cells migrated further into the anterior than the QR.d in wildtype animals (Fig. 3A), a phenotype that is also observed in lin-17/Fz and bar-1 mutants (Whangbo et al. 2000)."Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00004202Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
WBbt:0004086PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
WBbt:0004993PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
WBbt:0004054PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
WBPhenotype:0001278Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Remark"We found that 40/40 wild-type animals expressed the mab-5::lacZ reporter in the QL daughter cells (Fig. 3B). In animals that overexpress PRY-1, no mab-5::lacZ expression was observed in the QL.d (n = 40), indicating that PRY-1 overexpression inhibits mab-5 expression."Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00004202Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Phenotype_assayGenotypemuIs2 [mab-5::lacZ]Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000232Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Remark"The localization of other migratory neurons, such as the HSN and CAN neurons, was not affected (n = 26) by PRY-1 overexpression."Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00004202Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006827PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
WBPhenotype:0000470Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Remark"The localization of other migratory neurons, such as the HSN and CAN neurons, was not affected (n = 26) by PRY-1 overexpression."Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00004202Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006830PATO:0000460Paper_evidenceWBPaper00005278
Curator_confirmedWBPerson2987
Used_forExpr_patternExpr1896
InteractorWBInteraction000538501
ReferenceWBPaper00005278
SpeciesCaenorhabditis elegans