WormBase Tree Display for Transgene: WBTransgene00000317
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| WBTransgene00000317 | Public_name | cgc6123Is1 | |
|---|---|---|---|
| Summary | [bam-2::gfp] | ||
| Construction | Construct | WBCnstr00000317 | |
| Construction_summary | bam-2 expression clones were made in a pSK vector using a PCR-based approach to fuse the coding region of GFP and the 3'UTR from unc-54, amplified from pPD95.75, in-frame to the C-terminus of a full length bam-2 cDNA (yk593b12) to create bam-2::gfp. bam-2 promoter fragments were PCR amplified from N2 genomic DNA and cloned upstream of bam-2::gfp or into the multiple cloning site of pPD95.75. Promoter derivatives were sometimes obtained by restriction digest, for example using XbaI to generate xbaIp:bam-2::gfp. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2767 | |
| Reference | WBPaper00006123 | ||
| Species | Caenorhabditis elegans |
