WormBase Tree Display for Transgene: WBTransgene00000525

WBTransgene00000525 Public_name: gaIs37
  Summary: [EF1a::Dmek + hs::mpk-1]
  Synonym: [EF1alpha-D-mek(gf); hs-mpk-1(wt)]
    [Ef1a::Dmek hs::mpk-1]
    [hs-Dsor-1; hs-mpk-1(activated)]
  Construction: Construct: WBCnstr00000524
  Genetic_information: Integrated
    Phenotype: WBPhenotype:0000700 Paper_evidence: WBPaper00042320
          WBPaper00003200
        Curator_confirmed: WBPerson712
          WBPerson2987
        Remark: Hyperactive mpk-1 mutants, gaIs37, exhibited a Muv phenotype in 98.8% of animals (n= 322) at 24 deg C. Paper_evidence: WBPaper00042320
            Curator_confirmed: WBPerson712
          Table 1 Paper_evidence: WBPaper00003200
            Curator_confirmed: WBPerson2987
        Penetrance: High: 94% penetrance Paper_evidence: WBPaper00003200
              Curator_confirmed: WBPerson2987
        Caused_by_gene: WBGene00003401 Paper_evidence: WBPaper00042320
              WBPaper00003200
            Curator_confirmed: WBPerson712
              WBPerson2987
        EQ_annotations: Anatomy_term: WBbt:0006748 PATO:0000460 Paper_evidence: WBPaper00003200
                Curator_confirmed: WBPerson2987
          Life_stage: WBls:0000035 PATO:0000460 Paper_evidence: WBPaper00003200
                Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: To measure vulval induction in this strain, eggs from a gaIs37 strain were moved from 15 degrees Celsius to 25 degrees Celsius and allowed to develop at that temperature. Early L4 stage animals from the strain were picked from 25 degrees Celsius and observed by Nomarski photomicroscopy for the extent of vulval induction and for the fate of P12.p. In this experiment, we scored those animals with inductions at P3.p, P4.p or P8.p as "Muv" in Table 1 and those animals with no inductions at these cells as non-Muv, even though adult animals were not scored. Paper_evidence: WBPaper00003200
              Curator_confirmed: WBPerson2987
          Temperature: 25 Paper_evidence: WBPaper00003200
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001645 Paper_evidence: WBPaper00005245
        Curator_confirmed: WBPerson1754
        Remark: Increased protein degradation at restrictive temperature Paper_evidence: WBPaper00005245
            Curator_confirmed: WBPerson1754
        Caused_by_gene: WBGene00003401 Paper_evidence: WBPaper00005245
            Curator_confirmed: WBPerson1754
  Used_for: Interactor: WBInteraction000000713
  Associated_with: Strain: WBStrain00030720
      WBStrain00030755
  Reference (11)
  Species: Caenorhabditis elegans