WormBase Tree Display for Transgene: WBTransgene00000546
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| WBTransgene00000546 | Public_name | geIn3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [sir-2.1; rol-6] | ||||||||
| Construction | Construct | WBCnstr00000544 | |||||||
| Coinjection_other | rol-6 | ||||||||
| Integration_method | Gamma_irradiation | ||||||||
| Construction_summary | sir-2.1 refers to sequence R11A8.4. | ||||||||
| A 2.2 kb genomic fragment of sir-2.1 was injected into N2 along with the rol-6 transformation marker. | |||||||||
| Genetic_information | Integrated | ||||||||
| Phenotype | WBPhenotype:0000134 | Paper_evidence | WBPaper00036476 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Remark | Overexpression of SIR-2.1 results in decreased transcription of fat-7 and lbp-6 under both fed and fasted conditions, compared to control animals. | Paper_evidence | WBPaper00036476 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00036476 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| WBPhenotype:0001183 | Paper_evidence | WBPaper00036476 | |||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Remark | Increased SIR-2.1 activity in nematodes results in lower levels of stored fat, as measured by Sudan Black staining. | Paper_evidence | WBPaper00036476 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00036476 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00036476 | ||||
| Curator_confirmed | WBPerson712 | ||||||||
| Phenotype_not_observed | WBPhenotype:0000061 | Paper_evidence | WBPaper00040229 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Remark | Strain (LG100) carrying the integrated transgene array geIn3 [sir-2.1 rol-6(su1006)] (ref. 1) was long-lived; however, outcrossing (x5) of geIn3 to wild type (N2) abrogated the increase in longevity. | Paper_evidence | WBPaper00040229 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00040229 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Associated_with | Strain | WBStrain00007690 | |||||||
| WBStrain00007691 | |||||||||
| WBStrain00007692 | |||||||||
| WBStrain00024177 | |||||||||
| Reference | WBPaper00004566 | ||||||||
| WBPaper00025055 | |||||||||
| WBPaper00026947 | |||||||||
| WBPaper00027148 | |||||||||
| WBPaper00027338 | |||||||||
| WBPaper00027682 | |||||||||
| WBPaper00027763 | |||||||||
| WBPaper00029298 | |||||||||
| WBPaper00031034 | |||||||||
| WBPaper00033162 | |||||||||
| WBPaper00033401 | |||||||||
| WBPaper00035277 | |||||||||
| WBPaper00036140 | |||||||||
| WBPaper00036476 | |||||||||
| WBPaper00038209 | |||||||||
| WBPaper00040139 | |||||||||
| WBPaper00040184 | |||||||||
| WBPaper00040228 | |||||||||
| WBPaper00040229 | |||||||||
| WBPaper00040798 | |||||||||
| WBPaper00041514 | |||||||||
| WBPaper00045681 | |||||||||
| WBPaper00045785 | |||||||||
| WBPaper00048984 | |||||||||
| WBPaper00058832 | |||||||||
| Species | Caenorhabditis elegans |
