WormBase Tree Display for Transgene: WBTransgene00000621
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| WBTransgene00000621 | Public_name | hyEx80 | |
|---|---|---|---|
| Summary | [smo-1::gfp; rol-6] | ||
| Construction | Construct | WBCnstr00000619 | |
| Construction_summary | The SMO-1::GFP reporter is a PCR product amplified from C. elegans genomic DNA with the primers K12C11A1 (5'-GTCGAAAACACTGGAAATTGCTGT-3') and K12C11B (5'-AGTCGACCTGCAGGCATCCAAGCTTGAATCCGCCCAGCTGCTCTTG GTA-3') and then reamplified with internal primers and a GFP fragment of pPD95.75 to create a GFP fusion construct. The final product contains 1 Kb upstream of the smo-1 coding region, the entire coding region fused to GFP, and the 3' UTR of unc-54. Three transgenic lines of the translational fusion were analyzed. --precise ends. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr3449 | |
| Reference | WBPaper00024467 | ||
| Species | Caenorhabditis elegans |
