WormBase Tree Display for Transgene: WBTransgene00001651
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| WBTransgene00001651 | Public_name | oxIs103 | |
|---|---|---|---|
| Summary | [UNC-46::GFP; lin-15(+)] | ||
| Construction | Construct | WBCnstr00001640 | |
| Construction_summary | Clone = pKS9.1. GFP was inserted at the amino terminus of UNC-46 after the signal peptide. GFP was PCR-amplified such that it was flanked by PinAI restriction sites. This GFP fragment was subcloned into an endogenous PinAI site in exon 1 of the rescuing fragment for the unc-46 gene. This construct, generates an UNC-46 protein with an insertion of GFP after amino acid 24. The U46GFPntl construct rescued the unc-46 mutant phenotype, and the integrated array is oxIs103. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr4786 | |
| Reference | WBPaper00030788 | ||
| WBPaper00037731 | |||
| Species | Caenorhabditis elegans |
