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WormBase Tree Display for Transgene: WBTransgene00005025

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Name Class

WBTransgene00005025Public_nameWBPaper00032082Ex1
Summary[daf-1::daf-3]
ConstructionConstructWBCnstr00005002
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000012Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkOverexpression of daf-3 driven by daf-1 resulted in an increase in the number of daf-1(m40); daf-3(mgDf90) animals entering dauer.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000899Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Genotypedaf-1(m40); daf-3(mgDf90); WBPaper00032082Ex1[pdaf-1::daf-3]Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0000019Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkOverexpression of daf-3 driven by daf-1 resulted in a decrease in the pumping rate of daf-1(m40); daf-3(mgDf90) animals.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000899Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentTo minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature22Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Genotypedaf-1(m40); daf-3(mgDf90); WBPaper00032082Ex1[pdaf-1::daf-3]Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0001184Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000899Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentTo minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature22Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Genotypedaf-1(m40); daf-3(mgDf90)Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
ReferenceWBPaper00032082
SpeciesCaenorhabditis elegans