WormBase Tree Display for Transgene: WBTransgene00005029
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WBTransgene00005029 | Public_name | WBPaper00032082Ex5 | ||||||
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Summary | [tdc-1::daf-3] | |||||||
Construction | Construct | WBCnstr00005006 | ||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000012 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Overexpression of daf-3 driven by tdc-1 resulted in an increase in the number of daf-1(m40); daf-3(mgDf90) animals entering dauer. | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00000899 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00032082 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | daf-1(m40); daf-3(mgDf90); WBPaper00032082Ex5[ptdc-1::daf-3] | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000019 | Paper_evidence | WBPaper00032082 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Overexpression of daf-3 driven by daf-1 resulted in a decrease in the pumping rate of daf-1(m40); daf-3(mgDf90) animals. | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00000899 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | To minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype. | Paper_evidence | WBPaper00032082 | ||||
Curator_confirmed | WBPerson712 | |||||||
Temperature | 22 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | daf-1(m40); daf-3(mgDf90);WBPaper00032082Ex5[tdc-1::daf-3] | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001184 | Paper_evidence | WBPaper00032082 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00000899 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | To minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype. | Paper_evidence | WBPaper00032082 | ||||
Curator_confirmed | WBPerson712 | |||||||
Temperature | 22 | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | daf-1(m40); daf-3(mgDf90);WBPaper00032082Ex5[tdc-1::daf-3] | Paper_evidence | WBPaper00032082 | |||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00032082 | |||||||
Species | Caenorhabditis elegans |