Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00005248

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00005248Public_namezxIs3
Summary[unc-47p::ChR2(H134R)::YFP + lin-15(+)]
Synonym[Punc-47::chop-2(H134R)-yfp; lin-15(+)]
ConstructionConstructWBCnstr00005225
Construction_summary80 ng/ul unc-47p::ChR2(H134R)::YFP
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000644Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkAnimals became almost completely paralyzed after illumination (photoactivation of ChR2-YFP in GABAergic neurons). Paralysis was reduced within 3 sec of sustained illumination, and animals resumed movement, though they were uncoordinated and slow.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
WBPhenotype:0001478Curator_confirmedWBPerson712
Remark"...expression of the channelrhodopsin-2 (ChR2) gain-of-function mutant H134R in GABAergic neurons results in about 4 % of elongation, compared to about 3 % in ChrimsonSA expressing animals (Fig. 1C+D, Liewald et al., 2008). However, in contrast to ChR2, ChrimsonSA expressing animals do not show a decline of body length during stimulation.Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were treated with the chromophore all-trans-retinal (ATR), and a 10 s light stimulus.Curator_confirmedWBPerson712
WBPhenotype:0001802Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkAnimals became elongated, 104.1% of the initial length within ~350 ms of illumination (photoactivation of ChR2-YFP in GABAergic neurons). This body elongation was reversible by darkness, but did not attenuate under constant illumination.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001316Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkExogenous neurotransmitter evoked currents were no different from wild-type.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
WBPhenotype:0001318Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
RemarkThe frequency and amplitude of endogenous miniature postsynaptic currents of photostimulated ChR2-YFP containing GABAergic neurons were comparable to wild-type.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Caused_by_otherHuman channelrhodopsin-2 (H134R)Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Phenotype_assayTreatmentChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal.Paper_evidenceWBPaper00032190
Curator_confirmedWBPerson712
Associated_withStrainWBStrain00040910
Reference (13)
SpeciesCaenorhabditis elegans