WormBase Tree Display for Transgene: WBTransgene00007440
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WBTransgene00007440 | Public_name | WBPaper00036472Ex5 | |||||
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Summary | [Phsb-1::EAK-7(G2A)::GFP; rol-6(su1006)] | ||||||
Synonym | G2A EAK-7::GFP | ||||||
Construction | Construct | WBCnstr00007252 | |||||
Construction_summary | Translational fusion of an N-myristolation EAK-7 mutant. This N-myristoylation mutant was constructed using overlap extension PCR by incorporating nucleotide changes into the primers, resulting in mutation of glycine to alanine at residue 2 of EAK-7 (Alam et al., 2010). | ||||||
Genetic_information | Extrachromosomal | ||||||
Phenotype | WBPhenotype:0000436 | Paper_evidence | WBPaper00036472 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Remark | Mutation of the glycine residue (G2A) that is required for N-myristoylation resulted in diffuse cytoplasmic fluorescence whereas wild-type EAK-7::GFP localized to the plasma membrane | Paper_evidence | WBPaper00036472 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Caused_by_gene | WBGene00010671 | Paper_evidence | WBPaper00036472 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Reference | WBPaper00036472 | ||||||
Species | Caenorhabditis elegans |