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WormBase Tree Display for Transgene: WBTransgene00007440

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Name Class

WBTransgene00007440Public_nameWBPaper00036472Ex5
Summary[Phsb-1::EAK-7(G2A)::GFP; rol-6(su1006)]
SynonymG2A EAK-7::GFP
ConstructionConstructWBCnstr00007252
Construction_summaryTranslational fusion of an N-myristolation EAK-7 mutant. This N-myristoylation mutant was constructed using overlap extension PCR by incorporating nucleotide changes into the primers, resulting in mutation of glycine to alanine at residue 2 of EAK-7 (Alam et al., 2010).
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000436Paper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
RemarkMutation of the glycine residue (G2A) that is required for N-myristoylation resulted in diffuse cytoplasmic fluorescence whereas wild-type EAK-7::GFP localized to the plasma membranePaper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
Caused_by_geneWBGene00010671Paper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
ReferenceWBPaper00036472
SpeciesCaenorhabditis elegans