WormBase Tree Display for Transgene: WBTransgene00009211
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WBTransgene00009211 | Public_name | rmIs214 | |||||
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Summary | [pF25B3.3::257cAT3v1-1q75::yfp] | ||||||
Synonym | 275cAT3q75 | ||||||
Construction | Construct | WBCnstr00008897 | |||||
Integration_method | Gamma_irradiation | ||||||
Construction_summary | ATXN3 C-terminal YFP-tagged expression constructs (lacking 257aa from the amino terminal) were generated by PCR (using full-length constructs as a template) and cloned into the BamH I sites of PF25B3.3Q0::YFP, forming PF25B3.3::257cAT3q(n)::YFP. | ||||||
Genetic_information | Integrated | ||||||
Phenotype | WBPhenotype:0000306 | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | This ATXN3 C-terminal YFP-tagged expression construct containing a 75 polyQ tract (275cAT3q75) produces a heterogenous expression pattern in expressing neurons. FRAP analysis demonstrated differences in solubility of the protein among different neurons. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 lacking amino terminal 257 aa, with polyQ(75) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001405 | Paper_evidence | WBPaper00038396 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Unlike in animals expressing full-length AT3q14 (ataxin with polyQ(14) expansion) or YFP-alone, discrete foci formed. Unlike animals expressing AT3q75, protein aggregation was age-dependent. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 lacking amino terminal 257 aa, with polyQ(75) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001472 | Paper_evidence | WBPaper00038396 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals showed a significant reduction in the chemotaxis index when compared to N2 and control animals. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 C-terminal truncation with poly-Q(130) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Phenotype_not_observed (2) | |||||||
Reference | WBPaper00038396 | ||||||
Species | Caenorhabditis elegans |