WormBase Tree Display for Transgene: WBTransgene00010082
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WBTransgene00010082 | Public_name | WBPaper00040471Is1 | ||||||
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Summary | [PH20::(b)opsin; PH20::DsRed] | |||||||
Synonym | [N] (b)opsin | |||||||
WBPaper00040471_Is1 | ||||||||
Construction | Construct | WBCnstr00009730 | ||||||
Coinjection_other | PH20::DsRed | |||||||
Integration_method | UV | |||||||
Construction_summary | Neuronal expression of bovine opsin (NP_001014890). | |||||||
Genetic_information | Integrated | |||||||
Phenotype | WBPhenotype:0000644 | Paper_evidence | WBPaper00040471 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Worms expressing (b)opsin in neurons showed a sudden transient loss of motility both in crawling and thrashing assays upon light exposure after incubation with 9-cis-retinal. After incubation with all-trans retinal, locomotion was minimally affected. The reversible paralytic effect was abolished by the expression of pertussis toxin introduced by transgene. | Paper_evidence | WBPaper00040471 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | bovine opsin | Paper_evidence | WBPaper00040471 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00005297 | Paper_evidence | WBPaper00040471 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBMol:00004971 | Paper_evidence | WBPaper00040471 | ||||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001897 | Paper_evidence | WBPaper00040471 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Worms expressing (b)opsin in neurons showed a sudden transient loss of motility both in crawling and thrashing assays upon light exposure after incubation with 9-cis-retinal. After incubation with all-trans retinal, locomotion was minimally affected. The reversible paralytic effect was abolished by the expression of pertussis toxin introduced by transgene. | Paper_evidence | WBPaper00040471 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | bovine opsin | Paper_evidence | WBPaper00040471 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00005297 | Paper_evidence | WBPaper00040471 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBMol:00004971 | Paper_evidence | WBPaper00040471 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00040471 | |||||||
Species | Caenorhabditis elegans |