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WormBase Tree Display for Transgene: WBTransgene00010082

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Name Class

WBTransgene00010082Public_nameWBPaper00040471Is1
Summary[PH20::(b)opsin; PH20::DsRed]
Synonym[N] (b)opsin
WBPaper00040471_Is1
ConstructionConstructWBCnstr00009730
Coinjection_otherPH20::DsRed
Integration_methodUV
Construction_summaryNeuronal expression of bovine opsin (NP_001014890).
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000644Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
RemarkWorms expressing (b)opsin in neurons showed a sudden transient loss of motility both in crawling and thrashing assays upon light exposure after incubation with 9-cis-retinal. After incubation with all-trans retinal, locomotion was minimally affected. The reversible paralytic effect was abolished by the expression of pertussis toxin introduced by transgene.Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
Caused_by_otherbovine opsinPaper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005297Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
WBMol:00004971Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
WBPhenotype:0001897Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
RemarkWorms expressing (b)opsin in neurons showed a sudden transient loss of motility both in crawling and thrashing assays upon light exposure after incubation with 9-cis-retinal. After incubation with all-trans retinal, locomotion was minimally affected. The reversible paralytic effect was abolished by the expression of pertussis toxin introduced by transgene.Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
Caused_by_otherbovine opsinPaper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005297Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
WBMol:00004971Paper_evidenceWBPaper00040471
Curator_confirmedWBPerson712
ReferenceWBPaper00040471
SpeciesCaenorhabditis elegans