WormBase Tree Display for Transgene: WBTransgene00010146
expand all nodes | collapse all nodes | view schema
WBTransgene00010146 | Public_name | WBPaper00039855Ex14 | |||||
---|---|---|---|---|---|---|---|
Summary | [ceh-36p::crh-1dn] | ||||||
Synonym | WBPaper00039855_Ex14 | ||||||
Construction | Construct | WBCnstr00009790 | |||||
Construction_summary | Clone = pNYU21. We used pNYU2 as a template for the site-directed PCR mutagenesis (Quickchange mutagenesis kit, Stratagene), to create the cDNA encoding the dominant negative form of CRH-1 (CRH-1DN), which carries point mutation in its phosphorylation site Ser29. The fragment was digested by Acc65I and EcoRI and exchanged for the native crh-1 cDNA in pNYU10 and pNYU11. Expressed in AWC. | ||||||
Genetic_information | Extrachromosomal | ||||||
Phenotype_not_observed | WBPhenotype:0000478 | Paper_evidence | WBPaper00039855 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Wild-type animals expressing CRH-1DN in AWC neurons showed thermotactic behaviour similar to wild-type animals after the cultivation temperature change. | Paper_evidence | WBPaper00039855 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Dominant negative CRH-1 in AWC | Paper_evidence | WBPaper00039855 | ||||
Curator_confirmed | WBPerson712 | ||||||
Reference | WBPaper00039855 | ||||||
Species | Caenorhabditis elegans |