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WormBase Tree Display for Transgene: WBTransgene00010146

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Name Class

WBTransgene00010146Public_nameWBPaper00039855Ex14
Summary[ceh-36p::crh-1dn]
SynonymWBPaper00039855_Ex14
ConstructionConstructWBCnstr00009790
Construction_summaryClone = pNYU21. We used pNYU2 as a template for the site-directed PCR mutagenesis (Quickchange mutagenesis kit, Stratagene), to create the cDNA encoding the dominant negative form of CRH-1 (CRH-1DN), which carries point mutation in its phosphorylation site Ser29. The fragment was digested by Acc65I and EcoRI and exchanged for the native crh-1 cDNA in pNYU10 and pNYU11. Expressed in AWC.
Genetic_informationExtrachromosomal
Phenotype_not_observedWBPhenotype:0000478Paper_evidenceWBPaper00039855
Curator_confirmedWBPerson712
RemarkWild-type animals expressing CRH-1DN in AWC neurons showed thermotactic behaviour similar to wild-type animals after the cultivation temperature change.Paper_evidenceWBPaper00039855
Curator_confirmedWBPerson712
Caused_by_otherDominant negative CRH-1 in AWCPaper_evidenceWBPaper00039855
Curator_confirmedWBPerson712
ReferenceWBPaper00039855
SpeciesCaenorhabditis elegans