WormBase Tree Display for Transgene: WBTransgene00016091
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| WBTransgene00016091 | Public_name | uthEx556 | |
|---|---|---|---|
| Summary | [sur-5p::rpn-6 + myo-3p::GFP] | ||
| Synonym | [psur-5::rpn-6.1; pmyo-3::GFP] | ||
| Construction | Construct | WBCnstr00015657 | |
| Coinjection_other | pmyo-3::GFP | ||
| Construction_summary | Clone = pDV1 and pPD93_97. The rpn-6.1 C. elegans expression plasmid pPD95.77, from the Fire Lab kit, was digested with SphI and XmaI to insert 3.6 kilobases of the sur-5 promoter. The resultant vector was then digested with KpnI and EcoRI to excise GFP and insert a multi-cloning site containing KpnI, NheI, NotI, XbaI and EcoRI. F57B9.10.A (rpn-6.1) was PCR amplified from cDNA to include 59 XmaI and 39 XbaI restriction sites then cloned into the aforementioned vector. | ||
| Genetic_information | Extrachromosomal | ||
| Associated_with | Strain | WBStrain00000103 | |
| Reference | WBPaper00041480 | ||
| Species | Caenorhabditis elegans |
