WormBase Tree Display for Transgene: WBTransgene00017734
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| WBTransgene00017734 | Public_name | nIs308 | |
|---|---|---|---|
| Summary | [Pmec-7::csp-1B, Pmec-3::gfp] | ||
| Construction | Construct | WBCnstr00017126 | |
| Construction_summary | The Pmec-7::csp-1B plasmid (pDD002) was constructed by using PCR to amplify the csp-1B cDNA from pDD001 using the primers 5'-gcggctagcatgccgagaacggacgccaag and 5'-gcgccatggttacatcgaccttgaaaagtgcc, which incorporate the restriction sites NheI and NcoI, respectively. The csp-1B amplicon was digested with NheI and NcoI and then ligated into the vector pPD52.102, which encodes the mec-7 promoter. | ||
| Genetic_information | Integrated | ||
| Map | III | ||
| Reference | WBPaper00042142 | ||
| Species | Caenorhabditis elegans |
