WormBase Tree Display for Transgene: WBTransgene00019110
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| WBTransgene00019110 | Public_name | vizIs22 | |
|---|---|---|---|
| Summary | [Ppie-1::gfp::daf-2; unc-119 (+)] | ||
| Synonym | GFP::DAF-2 | ||
| Construction | Construct | WBCnstr00018458 | |
| Coinjection_other | unc-119(+) | ||
| Integration_method | Particle_bombardment | ||
| Construction_summary | To create a GFP::DAF-2 construct, the daf-2 coding region from the start codon to the translation stop was amplified as one PCR product of 5.5 Kb with KOD polymerase (Novagen, Madison, WI), using the following primers: 5' region-atgaatattgtcagatgtcggagacgacac and 3' region- tcagacaagtggatgatgctcattatcctc. The products were amplified as attB1 and attB2 products and recombined into the pDONR vector of the gateway system. The pDONR plasmid was then sequenced and recombined into the pID3.02 pie-1 GFP plasmid containing the unc-119 transformation marker. | ||
| Genetic_information | Integrated | ||
| Reference | WBPaper00044292 | ||
| WBPaper00050726 | |||
| Species | Caenorhabditis elegans |
