WormBase Tree Display for Transgene: WBTransgene00019820

WBTransgene00019820 Public_name: bkIs423
  Summary: [Psnb-1::hTDP-43(M337V); Pmyo-2::dsRED]
  Construction: Construct: WBCnstr00019148
    Coinjection_other: Pmyo-2::dsRED
    Integration_method: Gamma_irradiation
  Construction_summary: The marker transgene Pmyo-2::dsRED was used at a concentration of 10 ng/l as a coinjection marker as previously described (Guthrie et al., 2009). The TDP-43 construct was microinjected into N2 at a concentration of 160-180 ng/l as previously described (Kraemer et al., 2003) to produce worms with TDP-43 or green fluorescent protein (GFP) and dsRED fluorescent protein (RFP) transgenes as extrachromosomal arrays. Transgenes were integrated by exposing animals to a dose of 3200-3600 rad gamma rays and integrated strains were backcrossed to N2 at least twice.
    NOTE:genotype reported as [Psnb-1::hTDP-43; myo-2::dsRED] in Weeks et al., 2017.
  Genetic_information: Integrated
    Phenotype: WBPhenotype:0000643 Paper_evidence: WBPaper00042050
        Curator_confirmed: WBPerson712
        Remark: M337V animals have severe movement defects, including coiling, uncoordinated locomotion, and paralysis Paper_evidence: WBPaper00042050
            Curator_confirmed: WBPerson712
        Caused_by_other: human ALS-mutant M337V TDP-43 (M337V) Paper_evidence: WBPaper00042050
            Curator_confirmed: WBPerson712
  Associated_with: Strain: WBStrain00005045
  Reference: WBPaper00037845
    WBPaper00042050
    WBPaper00053884
    WBPaper00058962
    WBPaper00059979
  Species: Caenorhabditis elegans