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WormBase Tree Display for Transgene: WBTransgene00020475

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WBTransgene00020475	Public_name	laxIs004
	Summary	[mafr-1p::mafr-1-GFP::mafr-1 3'UTR]
	Construction	Construct	WBCnstr00020095
	Genetic_information	Integrated
		Phenotype	WBPhenotype:0000136	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	MAFR-1 overexpression results in increased expression of 1,317 genes, as determined by microarray analysis (Table S1).	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
					mafr-1 overexpression resulted in increased mRNA levels of the gene sod-3 (Figure S5E)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0000137	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	MAFR-1 overexpression results in reduced expression of the RNA Pol II target tbp-1 (Figure 1D).	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
					MAFR-1 overexpression results in reduced expression of 1,495 genes, as determined by microarray analysis (Table 1).	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
					MAFR-1 overexpression results in reduced expression of genes vit-2, vit-4, vit-5, and vit-6 (Figure 2B)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
					MAFR-1 overexpression results in reduced expression of genes pod-2 and fasn-1 (Figure 4B)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0000154	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	mafr-1 overexpression animals display a >50% reduction in fecundity as compared to wild-type controls (Figure 3A)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0000229	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	mafr-1 overexpression leads to an approximately 7% decrease in body area (Figure S1D)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0001183	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Overexpression of mafr-1 led to a significant 35% reduction of intracellular lipids (Figures 4E and 4F; Figure S4F), as determined by Nile red and Oil O red O staining	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0001278	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Oocytes in mafr-1 overexpression animals contained less intracellular VIT-2::GFP when compared to controls (Figure 2D).	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Phenotype_assay	Genotype	VIT-2::GFP	Paper_evidence	WBPaper00046188
							Curator_confirmed	WBPerson2987
			WBPhenotype:0002353	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Overexpression of mafr-1 resulted in a reduction of expression for many tRNAs (Figure 1B, S1B)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
		Phenotype_not_observed	WBPhenotype:0000033	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Figure S1E	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0000048	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	mafr-1 overexpression had no measurable effect on embryo viability as all eggs hatched (Figure 3B).	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0000114	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	mafr-1 overexpression did not affect mRNA expression levels of the gene tlf-1 (Figure S1F)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
					mafr-1 overexpression did not affect mRNA expression levels of the genes acs-11, acs-2, cpt-3, acdh-2, hacd-1, B0303.3, F08A8.4, T02G5.7, or atgl-1 (Figure S4C)	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0001006	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Figure S4I	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
			WBPhenotype:0001183	Paper_evidence	WBPaper00046188
				Curator_confirmed	WBPerson2987
				Remark	Figure 5E, S5B	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Caused_by_gene	WBGene00016622	Paper_evidence	WBPaper00046188
						Curator_confirmed	WBPerson2987
				Phenotype_assay	Genotype	daf-18(ok480)	Paper_evidence	WBPaper00046188
							Curator_confirmed	WBPerson2987
						daf-16(mu86)	Paper_evidence	WBPaper00046188
							Curator_confirmed	WBPerson2987
	Used_for	Expr_pattern	Expr12112
		Interactor	WBInteraction000525385
			WBInteraction000525388
	Associated_with	Strain	WBStrain00045500
	Reference	WBPaper00046188
	Species	Caenorhabditis elegans