WormBase Tree Display for Transgene: WBTransgene00020851
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| WBTransgene00020851 | Public_name | dzEx1240 | |
|---|---|---|---|
| Summary | [Pgcy-8::BirA::nrx-1; Pttx-3::AP::nlg-1; Punc-122::streptavidin::tagRFP; elt-2::GFP] | ||
| Synonym | iBLINC | ||
| vivoBLINC | |||
| Construction | Construct | WBCnstr00020409 | |
| WBCnstr00020411 | |||
| Coinjection_other | elt-2::GFP | ||
| Construction_summary | To develop a methodology that involves a trans-synaptic enzymatic reaction we adapted BLINC (Biotin Labeling of INtercellular Contacts), a technique established in cell culture (LIU et al. 2013) and developed it for transgenic use in C. elegans. BLINC is based on enzymatictransfer of biotin onto a fifteen amino acid peptide (the acceptor peptide, AP) by the E. coli biotin ligase BirA (LIU et al. 2013). The in vivo BLINC method seems to capture the directionality of synaptic connections and permits the quantification of synaptic patterns in a population of animals. To detect the biotinylated AP::NLG-1/neuroligin fusion in vivo we designed a monomeric streptavidin::tagRFP detector that we transgenically secreted into the extracellular space of C. elegans from six scavenger cells termed coelomocytes (Figure 1A,B, Figure S1A). We term this technique iBLINC, for in vivoBLINC. | ||
| Genetic_information | Extrachromosomal | ||
| Reference | WBPaper00046709 | ||
| Species | Caenorhabditis elegans |
