WormBase Tree Display for Transgene: WBTransgene00021360
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| WBTransgene00021360 | Public_name | tvEx158 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [hsp-16::ULP-2;HMR-1::GFP;rol-6] | ||||||||
| Construction | Construct | WBCnstr00020937 | |||||||
| WBCnstr00006080 | |||||||||
| Laboratory | NX | ||||||||
| Genetic_information | Extrachromosomal | ||||||||
| Phenotype | WBPhenotype:0000436 | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | Overexpression of ULP-2, which prevents HMR-1 sumoylation (Figures 5F and 5G), showed HMR-1::GFP basolateral retention (Figures 6A-6C). | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00006737 | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Life_stage | WBls:0000003 | PATO:0000460 | Paper_evidence | WBPaper00048594 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| GO_term | GO:0045176 | PATO:0000460 | Paper_evidence | WBPaper00048594 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001908 | Paper_evidence | WBPaper00048594 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | ULP-2-overexpressing embryos were arrested during ventral enclosure (31%, n = 128). | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00006737 | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Life_stage | WBls:0000003 | PATO:0000460 | Paper_evidence | WBPaper00048594 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| GO_term | GO:0009790 | PATO:0000460 | Paper_evidence | WBPaper00048594 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0002362 | Paper_evidence | WBPaper00048594 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | To determine whether HMR-1 is sumoylated in vivo, authors immunoprecipitated extracts of HMR-1::GFP embryos with anti-GFP antibody followed by western blotting with anti-SUMO antibody (Figure 5F). Sumoylated HMR-1 was undetectable in extracts of HMR-1::GFP embryos in which ULP-2 was overexpressed via a heat shock promoter (Figure 5F, lane 5, and Figure 5G). | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00006737 | Paper_evidence | WBPaper00048594 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Life_stage | WBls:0000003 | PATO:0000460 | Paper_evidence | WBPaper00048594 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| GO_term | GO:0033233 | PATO:0000460 | Paper_evidence | WBPaper00048594 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Associated_with | Strain | WBStrain00044256 | |||||||
| Reference | WBPaper00048594 | ||||||||
| Species | Caenorhabditis elegans |
