Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00023139

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00023139Public_namezfEx8
SummaryPlgc-55::LGC-55 cation-I
ConstructionConstructWBCnstr00022953
LaboratoryQW
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000315Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Remark"In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00013746Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Phenotype_assayGenotypelgc-55(tm2913)Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBPhenotype:0001005Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Remark"In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00013746Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Phenotype_assayGenotypelgc-55(tm2913)Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBPhenotype:0002366Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Remark"In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100). Strikingly, transgenic animals that expressed the LGC-55 cation channel variants contracted their neck muscles in response to touch (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -11 1.6 μm, n = 32; Plgc-55::LGC-55 cation-II (zfEx40): Δhead = -14 0.009 mm [sic], n = 26), and the average reversal length was markedly reduced (Plgc-55::LGC-55 cation-I (zfEx8): 1.57 0.1 body bends, n = 100; Plgc-55::LGC-55 cation-II (zfEx40): 1.22 0.1 body bends, n = 100) (Fig 6A and 6C). Furthermore, transgenic LGC-55 cation animals displayed ratchety backward locomotion, often pausing during their reversal (S4 Movie). In contrast to animals expressing the LGC-55 anion, which made long spontaneous reversals, LGC-55 cation animals predominantly make short reversals, and the number of spontaneous reversals is increased (S5 Fig)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00013746Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Phenotype_assayGenotypelgc-55(tm2913)Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBPhenotype:0002399Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Remark"Exogenous tyramine induced neck muscle relaxation and lengthening of the head in wild-type (LGC-55 anion) animals and lgc-55 null mutant animals that express a rescuing LGC-55 anion transgene (wild type: Δhead = 10 6 μm, n = 68; Plgc-55:LGC-55(zfEx2): Δhead = 11 4 μm, n = 75) (Fig 5A and 5B). Head movements persisted in lgc-55 mutants [19], with no significant change in head length (lgc-55(tm2913): Δhead = 3 13 μm, n = 65). In contrast, transgenic animals that expressed the engineered LGC-55 cation-I or LGC-55 cation-II channel under control of the native promoter had a hypercontracted and shortened head length in response to exogenous tyramine (Plgc-55::LGC-55 cation-I (zfEx8): Δhead = -15 2 μm, n = 49; Plgc-55::LGC-55 cation- II (zfEx40): Δhead = -28 2 μm, n = 49) (Fig 5A and 5B)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
"In wild-type animals, exogenous tyramine also induced long backward runs preceding immobilization through the LGC-55 mediated inhibition of the AVB premotor interneurons that drive forward locomotion (wild type: Δfwd-bwd = -8.46 2.98 body bends, n = 40) (Fig 5A and 5D, S1 Movie) [19]. Backward locomotion was further increased in transgenic animals that expressed the LGC-55 anion under control of its endogenous promoter (Plgc-55:LGC-55 (zfEx2): Δfwd-bwd = -28.5 3.3 backward body bends, n = 29). lgc-55 null mutants did not make long reversals when exposed to exogenous tyramine (lgc-55(tm2913): Δfwd-bwd = 2.58 0.9 body bends, n = 34). In sharp contrast, LGC-55 cation animals exhibit long forward runs (Plgc-55::LGC-55 cation-I (zfEx8): Δfwd-bwd = 46.5 8.4 body bends, n = 28; Plgc-55::LGC-55 cation-II (zfEx40): Δfwd-bwd = 80.6 9.5 body bends, n = 39), which continued for an extended period of time (Fig 5A, 5C, and 5D; S2 Movie)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00013746Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00001396Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Phenotype_assayGenotypelgc-55(tm2913)Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
ReferenceWBPaper00048522
SpeciesCaenorhabditis elegans