WormBase Tree Display for Transgene: WBTransgene00027258
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WBTransgene00027258 | Summary | [Phsp::HBx; Psur-5::SUR-5::GFP] | ||||||
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Construction | Construct | WBCnstr00015548 | ||||||
Coinjection | WBCnstr00042119 | |||||||
Construction_summary | The PhspHBx constructs (at 25 ng/μL each) were injected into animals with the appropriate genetic background, using Psur-5GFP (25 ng/μL) as a coinjection marker, which directs GFP expression in all somatic cells in most developmental stages (6). | |||||||
Genetic_information | Phenotype | WBPhenotype:0000050 | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "To assess the activities of HBx inC. elegans, we induced global expression of HBx under the control of the C. elegans heat-shock promoters (PhspHBx). Expression of HBx in C. elegans had deleterious effects, leading to a high percentage of embryonic lethality (Fig. 1A and B)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Penetrance | High | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000183 | Paper_evidence | WBPaper00041673 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Approximately 97% of PhspHBx transgenic embryos did not hatch and were invariably deformed (Fig. 1A and B). Many contained large vacuoles that resembled necrotic cells (Fig. 1A, arrowheads) and raised discs characteristic of apoptotic cells (Fig. 1A, arrows)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000351 | Paper_evidence | WBPaper00041673 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Approximately 97% of PhspHBx transgenic embryos did not hatch and were invariably deformed (Fig. 1A and B). Many contained large vacuoles that resembled necrotic cells (Fig. 1A, arrowheads) and raised discs characteristic of apoptotic cells (Fig. 1A, arrows)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Penetrance | High | 97% | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001136 | Paper_evidence | WBPaper00041673 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Approximately 97% of PhspHBx transgenic embryos did not hatch and were invariably deformed (Fig. 1A and B). Many contained large vacuoles that resembled necrotic cells (Fig. 1A, arrowheads) and raised discs characteristic of apoptotic cells (Fig. 1A, arrows)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Penetrance | High | 97% | Paper_evidence | WBPaper00041673 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001395 | Paper_evidence | WBPaper00041673 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Approximately 97% of PhspHBx transgenic embryos did not hatch and were invariably deformed (Fig. 1A and B). Many contained large vacuoles that resembled necrotic cells (Fig. 1A, arrowheads) and raised discs characteristic of apoptotic cells (Fig. 1A, arrows)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001397 | Paper_evidence | WBPaper00041673 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Approximately 97% of PhspHBx transgenic embryos did not hatch and were invariably deformed (Fig. 1A and B). Many contained large vacuoles that resembled necrotic cells (Fig. 1A, arrowheads) and raised discs characteristic of apoptotic cells (Fig. 1A, arrows)." | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_other | hepatitis B virus protein HBx | Paper_evidence | WBPaper00041673 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Reference | WBPaper00041673 | |||||||
Species | Caenorhabditis elegans |