WormBase Tree Display for Antibody: WBAntibody00000694
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| WBAntibody00000694 | Summary | Polyclonal rat antibody. Final bleeds of one rat was purified using the 6xHis-KLP-18 fragment conjugated to AminoLink Plus (Pierce). | ||
|---|---|---|---|---|
| Public_name | [cgc6197]::anti-KLP-18_b | |||
| Gene | WBGene00002228 | |||
| Isolation | Location | SS | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | PCR was used to amplify a 1.2 kb fragment of yk167g6, encoding the C-terminal 425 aa of KLP-18 (PARMA....TTKP): forward: 5-GAGGATCCCCTGCACGAATGGCTTTGTT-3, reverse: 5-ATGCGGTACCTGGCTTTGTGGTCTGACT-3. The fragment was cloned in frame into the 6xHis-tag expression vector pQE-30 (Qiagen), transformed, and induced in E. coli strain M15[pREP4] (Qiagen). Recombinant protein was purified on Ni-NTA matrix (Qiagen) and sent to Eurogentec for immunization. | ||
| Animal | Rat | |||
| Expr_pattern | Expr2632 | |||
| Reference | WBPaper00006197 | |||
| WBPaper00050038 | ||||
| WBPaper00046862 |
