WormBase Tree Display for Antibody: WBAntibody00000828
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| WBAntibody00000828 | Summary | Rabbit polyclonal antibody against MUT-7 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc7049]:mut-7 | |||
| Gene | WBGene00003504 | |||
| Isolation | Original_publication | WBPaper00024699 | ||
| Location | NL | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | The C-terminal part of mut-7 (amino acids 727 to 910) was cloned into pET23 (Novagen) and expressed in E.coli BL21. Recombinant proteins were purified using Ni-NTA-agarose beads (Qiagen). Protein was purified under denaturing conditions, and was refolded by dialysis to phosphate-buffered saline (PBS). This protein was used for immunization of rabbits. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr12342 | |||
| Reference | WBPaper00024699 |
