WormBase Tree Display for Antibody: WBAntibody00002753
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| WBAntibody00002753 | Summary | A rabbit phospho-specific polyclonal antibody was generated by Abmart using the phosphopeptide C-QFDR(pS)FILAS encompassing the S269 site in SYP-4. Two peptides were synthesized: An antigen peptide with phosphoserine C-QFDR(pS)FILAS and a control peptide without phosphorylation (C-QFDRSFILAS). Serum harvested from the rabbits immunized with the antigen peptides went through two rounds of affinity purification. First, serum was passed through a column to which the antigenic peptide was coupled in order to isolate phospho-specific SYP-4 antibodies. The eluate from the first column was then passed through a second column to which the control peptide was coupled and flow through was collected. This second step was done to remove any non-phosphorylated SYP-4 antibodies. An ELISA titer of ≥ 1:50,000 against the modified peptide and a modified/unmodified titer ratio of ≥ 8 were used as validation criteria. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00050992]::anti-phospho-SYP-4 | |||
| Gene | WBGene00019247 | |||
| Isolation | Original_publication | WBPaper00050992 | ||
| Location | CV | |||
| Clonality | Polyclonal | |||
| Antigen | Peptide | Two peptides were synthesized: An antigen peptide with phosphoserine C-QFDR(pS)FILAS and a control peptide without phosphorylation (C-QFDRSFILAS). | ||
| Animal | Rabbit | |||
| Reference | WBPaper00050992 | |||
| WBPaper00055540 | ||||
| WBPaper00053443 |
