WormBase Tree Display for Construct: WBCnstr00008730
expand all nodes | collapse all nodes | view schema
WBCnstr00008730 | Summary | [pcol-12::mCherry::STA-2] | |
---|---|---|---|
Driven_by_gene | WBGene00000601 | ||
Gene | WBGene00010251 | ||
Fusion_reporter | mCherry | ||
Construction_summary | This construct was obtained by multi-site recombinational gateway cloning according to manufacturer's manual (Invitrogen). A 1067bp fragment upstream of the col-12 start site was amplified together with recombination sequences and BP and cloned into the pDONRP4-P1R vector giving rise to pENTR-5' element. The sta-2 genomic fragment comprising its 3' UTR was amplified together with recombination tails and BP and cloned into the pDONR P2R-P3 vector, giving rise to pENTR-3' element. The produced pENTR vectors together with pENTR-mCherry were used in a multi-partite LR reactions into the pJPDest R4R3 vector. | ||
Used_for | Transgene_construct | WBTransgene00009041 | |
Reference | WBPaper00038424 |