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WormBase Tree Display for Construct: WBCnstr00008730

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Name Class

WBCnstr00008730Summary[pcol-12::mCherry::STA-2]
Driven_by_geneWBGene00000601
GeneWBGene00010251
Fusion_reportermCherry
Construction_summaryThis construct was obtained by multi-site recombinational gateway cloning according to manufacturer's manual (Invitrogen). A 1067bp fragment upstream of the col-12 start site was amplified together with recombination sequences and BP and cloned into the pDONRP4-P1R vector giving rise to pENTR-5' element. The sta-2 genomic fragment comprising its 3' UTR was amplified together with recombination tails and BP and cloned into the pDONR P2R-P3 vector, giving rise to pENTR-3' element. The produced pENTR vectors together with pENTR-mCherry were used in a multi-partite LR reactions into the pJPDest R4R3 vector.
Used_forTransgene_constructWBTransgene00009041
ReferenceWBPaper00038424