WormBase Tree Display for Construct: WBCnstr00010481
expand all nodes | collapse all nodes | view schema
| WBCnstr00010481 | Other_name | Expr1844_Ex | |
|---|---|---|---|
| Summary | [mig-15::gfp] | ||
| Driven_by_gene | WBGene00003247 | ||
| Gene | WBGene00003247 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [mig-15::gfp] translational fusion, full-length mig-15::gfp reporter construct that partially rescues mig-15(rh148). To generate a rescuing mig-15::gfp construct, two fragments were coinjected to generate stable transgenic animals: a 3.8-kb fragment corresponding to part of the mig-15 gene up to its last codon obtained after PCR amplification and cloning in frame into the GFP vector pPD95.75; and a 13-kb fragment corresponding to the rest of the gene and its putative promoter (overlap of 2 kb with the previous fragment) obtained after PCR amplification. | ||
| Clone | pPD95.75 | ||
| Used_for | Transgene_construct | WBTransgene00027716 | |
| Reference | WBPaper00005218 |
