WormBase Tree Display for Construct: WBCnstr00017724
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| WBCnstr00017724 | Other_name | Expr10770_Ex | |
|---|---|---|---|
| Summary | [Pacr-7::GFP; Pacr-7::acr-7::GFP] | ||
| Driven_by_gene | WBGene00000046 | ||
| Gene | WBGene00000046 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | To generate a Pacr-7::GFP transcriptional fusion construct, the putative promoter region was PCR-amplified, including 1.3-kb upstream of the acr-7 gene, using DNA prepared from adult animals. This clone was digested with PstI and XmaI restriction enzymes and ligated into the GFP vector pPD95.75. To generate a Pacr-7::acr-7::GFP translational fusion construct, the same acr-7 promoter sequence along with 4.3-kb of acr-7 genomic sequence was PCR-amplified, omitting the native stop codon, using DNA prepared from adult animals. This clone was digested with PstI and KpnI restriction enzymes, sub-electroporated into pCR-XL-TOPO (Invitrogen, Cat: K4700-10), and then subcloned into pPD95.75. -- precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00018339 | |
| Reference | WBPaper00042091 |
