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WormBase Tree Display for Construct: WBCnstr00018793

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Name Class

WBCnstr00018793Other_nameExpr11350_Ex
Summary[inx-1::GFP]
Driven_by_geneWBGene00002123
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summary[inx-1::GFP] transcriptional fusion. Plasmid for inx-1 (wp1264): a promoter sequence before the translation initiation site was amplified by PCR with genomic DNA as a template, and used to replace Pmyo-3 in pPD118.20 by cutting with Pst1 and BamH1 restriction enzymes. The sequences of the PCR primers were TGTCTCTGCTTTGCACGATT (sense) and attggatccGGCGGACAAGAACTGCAAT (antisense).
ClonepPD118.20
Used_forTransgene_constructWBTransgene00031466
ReferenceWBPaper00044311
Remark[inx-1::GFP] transcriptional fusion. Plasmid for inx-1 (wp1264): a promoter sequence before the translation initiation site was amplified by PCR with genomic DNA as a template, and used to replace Pmyo-3 in pPD118.20 by cutting with Pst1 and BamH1 restriction enzymes. The sequences of the PCR primers were TGTCTCTGCTTTGCACGATT (sense) and attggatccGGCGGACAAGAACTGCAAT (antisense).