WormBase Tree Display for Construct: WBCnstr00021081
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WBCnstr00021081 | Public_name | pBG-208 |
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Summary | [Phsp-16.2::RPM-1::GFP] | |
Driven_by_gene | WBGene00002016 | |
Gene | WBGene00004457 | |
Fusion_reporter | GFP | |
Construction_summary | Plasmids were generated using standard techniques including polymerase chain reaction, restriction endonuclease digestion (New England BioLabs), T4 ligase Cloning (New England BioLabs), and TOPO-TA and Gateway Cloning (Life Technologies). Promoter consists of 392 bp of genomic DNA upstream of the hsp-16.2 start codon and the RPM-1::GFP and 3' UTR sequence from pBG-46 (Opperman and Grill 2014). | |
Reference | WBPaper00048707 |