WormBase Tree Display for Construct: WBCnstr00023078
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| WBCnstr00023078 | Summary | [Pmex-5::MODC PEST:GFP:H2B::hbl-1 3'UTR Cbr-unc-119(+)] | |
|---|---|---|---|
| Driven_by_gene | WBGene00003230 | ||
| UTR_3 | WBGene00001824 | ||
| Fusion_reporter | GFP | ||
| Purification_tag | Histone H2B | ||
| Selection_marker | Cbr-unc-119(+) | ||
| Construction_summary | The 3'UTR sequences were amplified from worm genomic DNA using UTR-specific primers flanked with atB2R and attB3 sequences for Gateway cloning. BP Clonase II was then used to clone the sequences into pDONRP2RP3. Multisite gateway cloning was then used to fuse this donor construct with pCM1.111 (construct carrying the mex-5 promoter) and pBMF2.7 (construct carrying MODC PEST:GFP:H2B). The PEST domain in the GFP construct helped reduce the half-life of GFP to prevent expansion of GFP expression to regions where it is not expressed due to its high stability (Farley and Ryder, 2012). LR Clonase II plus was used to integrate the fusion constructs into pCFJ150. | ||
| Used_for | Transgene_construct | WBTransgene00023228 | |
| Reference | WBPaper00049723 |
