WormBase Tree Display for Expr_pattern: Expr14411
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| Expr14411 | Expression_of | Gene | WBGene00009264 |
|---|---|---|---|
| Expression_data | GO_term | GO:0005769 | |
| GO:0005783 | |||
| GO:0005794 | |||
| Subcellular_localization | In wild-type animals, mCherry-SAC-1 labels ER-like structures in the deep cytosol and punctate structures near the basolateral plasma membrane, and GFP-tagged ARF-6 resides in basolateral tubules and puncta (Shi et al., 2012; Gleason et al., 2014). mCherry-SAC-1 and ARF-6-GFP had significant colocalization in basolateral puncta. RAB-5/Rab5 is a marker of early endosomes (Nielsen et al., 2000; Chen et al., 2006; Murray et al., 2016). We compared the localization of SAC-1 with RAB-5 and observed that mCherry-SAC-1 overlapped well with GFP-RAB-5 on endosomal structures. Previous studies showed that recycling regulator RAB-10 partially colocalizes with RAB-5 on early endosomes, where it appears to promote the maturation of early endosomes to recycling endosomes (Chen et al., 2006; Shi et al., 2010). In our study, SAC-1 and RAB-10 had significant overlap in cytosolic punctate structures. We also compared the localization of SAC-1 with other endosomal markers. SAC-1 failed to significantly overlap with the late endosome marker RAB-7. Sac1p/ hSAC1 has been reported to reside in the ER and Golgi, where it participates in the regulation of PI(4)P pool (Kochendrfer et al., 1999; Blagoveshchenskaya et al., 2008; Bajaj Pahuja et al., 2015; Chung et al., 2015). As expected, we observed a partial colocalization between SAC-1 and the TGN marker AMAN-2. There was extensive overlap between the ER marker SP12-GFP and mCherry-SAC-1 in patch-like structures. | ||
| Type | Reporter_gene | ||
| Reference | WBPaper00053879 | ||
| Transgene | WBTransgene00026080 |
