WormBase Tree Display for Expr_pattern: Expr2247
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Expr2247 | Expression_of | Gene | WBGene00003735 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003735 | |||
Expression_data | Anatomy_term | WBbt:0004510 | Certain | |
WBbt:0004511 | Certain | |||
WBbt:0005451 | Certain | |||
WBbt:0005753 | Certain | |||
WBbt:0005767 | Certain | |||
WBbt:0006748 | Certain | |||
GO_term | GO:0031090 | |||
Subcellular_localization | NHX-8 appeared to be associated with intracellular membranes. The NHX-8::GFP protein was distributed in the seam cells in a vesicular pattern that excluded the nucleus, with some underlying signal contributed by weakly fluorescent hypodermal syncitia. | |||
Type | Reporter_gene | |||
Pattern | In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. NHX-8::GFP was expressed as expected in the seam cells but was also expressed in the pharyngeal muscles, the pharyngeal-intestinal and intestinal-rectal valve cells, and in cells of the vulva. Expression in these additional cells may have been a result of promoter or enhancer elements in the first intron of the nhx-8 gene that are not present in the transcriptional fusion construct. Alternatively, the shorter promoter used in the translational reporter construct (1.5 versus 4 kb) may lack sequences that normally repress expression in these cells. See Expr2237 for expression pattern observed with transcriptional fusion construct. | |||
Picture | WBPicture0000008208 | |||
Reference | WBPaper00005421 | |||
Transgene | WBTransgene00027959 |