WormBase Tree Display for Expr_pattern: Expr2269
expand all nodes | collapse all nodes | view schema
| Expr2269 | Expression_of | Gene | WBGene00001597 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001597 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000005 | ||||
| WBls:0000010 | ||||
| WBls:0000057 | ||||
| Anatomy_term | WBbt:0005784 | Certain | ||
| WBbt:0007850 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0043186 | ||||
| Subcellular_localization | The GLD-3 subcellular distribution was investigated by immunocytochemistry. Similar results were obtained using either anti-GLD-3 or anti-GLD-3L. In most experiments, antibodies to the P granule protein PGL-1 were used to highlight germ cells and P granules. The data support three main conclusions. First, GLD-3 protein is predominantly cytoplasmic. GLD-3 was found in germline cytoplasm throughout development in both sexes and in the cytoplasm of early embryos. Second, GLD-3 distribution is controlled spatially and temporally. In adult hermaphrodites, GLD-3 was detectable in the transition zone (TZ), where germ cells enter meiotic prophase; it was fainter in the early mitotic region and in pachytene germ cells; however, as germ cells entered diakinesis, GLD-3 became abundant. During spermatogenesis, GLD-3 was present in primary spermatocytes, but not in secondary spermatocytes or mature sperm. A similar pattern was observed in males. Third, GLD-3 colocalizes with P granules. GLD-3 was found in the cytoplasm of early embryos, and colocalized with P granules from the 1-cell stage through the 64-cell stage; beyond the 100-cell stage of embryogenesis, GLD-3 was no longer detected. GLD-3 also appeared in particles that were near but not coincident with P granules. In the germline, GLD-3 was found both diffusely as well as in a more granular form. The granular GLD-3 was found primarily at the cytoplasmic side of the nuclear boundary and often colocalized with PGL-1. Mutant embryos derived from gld-3(q730) homozygous mothers could contain apparently normal P granules that appeared to segregate normally to a single blastomere; however, these gld-3 mutant embryos had no detectable GLD-3, consistent with the idea that this mutant removes most gld-3 activity. | |||
| Type | Antibody | Two polyclonal antibodies. One, called anti-GLD-3, was raised to the N-terminal 23 amino acids of GLD-3 and recognized both GLD-3L and GLD-3S; the second, called anti-GLD-3L, was raised to a 368 amino acid frag ment found in GLD-3L, but not GLD-3S, and recognized only GLD-3L. | ||
| Pattern | Wild-type adults of either sex expressed both GLD-3L and GLD-3S. By contrast, adult glp-1(ts) hermaphrodites, which have virtually no germline, had no detectable GLD-3, consistent with the idea that gld-3 is expressed specifically in the germline. Furthermore, the deletion mutant, gld-3(q730), made no detectable GLD-3, confirming specificity of the antibodies. | |||
| Picture | WBPicture0000009912 | |||
| Reference | WBPaper00005566 | |||
| Antibody_info | WBAntibody00000574 | |||
| WBAntibody00000575 |
