WormBase Tree Display for Expr_pattern: Expr2295
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| Expr2295 | Expression_of | Gene | WBGene00004857 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004857 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | C-terminal construct: Nuclear accumulation in all of these tissues is strong. This nuclear localization does not depend on the activity of sma-6, however. When the integrated N-terminal construct array (qcIs6) was crossed into sma-4(e729) or sma-2(e502) mutant backgrounds, the nuclear localization did not change significantly. When the array is crossed into sma-6(wk7) mutants, the protein became evenly distributed between the cytoplasm and the nucleus in many but not all animals. Thus, the nuclear accumulation of SMA-3::GFP is enhanced by but not dependent on activation by the type I receptor. Determining whether this extensive nuclear localization is characteristic of the endogenous SMA-3 protein must await the development of SMA-3 antibodies. | |||
| Type | Reporter_gene | |||
| Pattern | C-terminal construct: Expression begins late in embryogenesis, and continues through larval stages into adulthood. In larvae, expression is strong in the hypodermis, pharynx and intestine. sma-3 expression in the hypodermis is seen throughout the large hypodermal syncytium hyp7, but not in the lateral hypodermal blast cells (the seam cell). | |||
| Expression of the N-terminal construct is similar, although much weaker, even after integration. Again, the nuclear fluorescence is prominent in the pharynx, intestine and hypodermis. | ||||
| Picture | WBPicture0000007519 | |||
| Reference | WBPaper00005567 | |||
| Transgene | WBTransgene00001868 |
