WormBase Tree Display for Expr_pattern: Expr8620
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| Expr8620 | Expression_of | Gene | WBGene00007975 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007975 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0005737 | |||
| GO:0043186 | ||||
| Subcellular_localization | In addition to a diffused cytoplasmic localization, a granular GLS-1 staining was observed in the most distal mitotic region and in germ cells entering meiotic diplotene in the proximal germ line. Almost all GLS-1 granules overlap with P granules, although GLS-1 intensities varied with respect to PGL-1 and GLH-2 intensities among P granules. This may reflect a selective enrichment of GLS-1 in P granules. | |||
| Type | Antibody | |||
| Pattern | GLS-1 is expressed in all germ cells, with the exception of spermatocytes. In the adult hermaphrodite, GLS-1 is present in the mitotic region, accumulates during early stages of meiotic prophase I and is slightly less abundant in maturing proximal oocytes. | |||
| Picture | WBPicture0000007378 | |||
| Remark | Picture: Fig 2. | |||
| The ubiquitous GLS-1 expression overlaps the ubiquitous expression of GLD-3, yet GLS-1 and GLD-3 differ in their expression pattern during early and late meiotic prophase I. As a specificity control, authors stained germ lines extruded from gls-1 mutant animals. No immunoreactivity was observed with the monoclonal GLS-1 antibody, although GLD-3 and PGL-1 were readily detected. We conclude that GLS-1 expression and subcellular localization in the germ line is dynamic and largely overlaps with GLD-3. GLS-1 is present in oocytes, which suggests a possible maternal role for gls-1. | ||||
| Reference | WBPaper00033153 | |||
| Antibody_info | WBAntibody00001927 | |||
| WBAntibody00001928 |
