WormBase Tree Display for Interaction: WBInteraction000502675
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| WBInteraction000502675 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00015646 | Interactor_type | Trans_regulated | |
| Expr_pattern | Expr3696 | ||||
| Transgene | WBTransgene00000986 | ||||
| Construct | WBCnstr00000978 | ||||
| WBGene00002285 | Interactor_type | Trans_regulator | |||
| WBGene00003312 | Interactor_type | Trans_regulator | |||
| Variation_interactor | WBVar00088922 | Interactor_type | Trans_regulator | ||
| WBVar00250308 | Interactor_type | Trans_regulator | |||
| Interaction_summary | Wild-type animals expressed a pulse of mlt-10::GFP before each of the four larval molts, but is not expressed in adults that no longer molt. The let-7(mg279) mir-84(tm1304) double mutants expressed an extra pulse of GFP, at levels comparable to those of wild-type animals late in the fourth larval stage, as judged by visual inspection and also by western analysis. | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [mlt-10::gfp-PEST], transcriptional fusion. | ||||
| Transgene | |||||
| Western | Western blot showing GFP in protein extracts from late L4-stage or gravid adults cultivated, respectively. | ||||
| Regulation_level | Post_transcriptional | ||||
| Regulation_result | Negative_regulate | Life_stage | WBls:0000041 | ||
| Anatomy_term | WBbt:0003672 | ||||
| Paper | WBPaper00028747 | ||||
| Remark | mir-84 acts together with let-7 to promote the cessation of molting. |
