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WBPicture0000007506DescriptionFigure 3. Embryonic and neuronal expression of PTP-3. (A-F) Expression of PTP-3B-GFP in enclosure stage embryos. Animals are of genotype juEx189; GFP is visualized by anti-GFP immunostaining (green), animals are also immunostained with MH27 to visualize adherens junctions (red). (A-C) A lateral confocal projection of an embryo prior to epidermal enclosure. The dorsal sheet of epidermal cells is visualised by MH27 staining; PTP-3B::GFP expression is widespread in surface cells; expression in ventral neuroblasts is marked in A (arrowhead). (D-F) A post-gastrulation embryo; medial confocal section showing widespread expression of PTP-3B::GFP at cell surfaces in epidermal, neuronal, pharyngeal, muscle, and endodermal tissue layers. (G) Wild-type L1 larva stained with anti-PTP-3 antibodies and MH27. Nerve ring staining is marked by the arrowhead. (H,I) PTP-3B-GFP expression in L1 (H) and adult (I) (juEx189), stained with anti-GFP antibodies; note intense nerve ring expression (arrowhead in I) and staining in neuronal processes. Scale bar, 10 um (A-F); 30 um (G, H); 100 um (I).
NameF3.large.jpg
DepictExpr_patternExpr1887
AnatomyWBbt:0003679
WBbt:0006749
WBbt:0007028
Cellular_componentGO:0005912
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2002
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00005259