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WBPicture0000008167 | Description | Figure 5. Immunostaining of VHA-5. Embryos at different cleavage stages (A, 4 cells; B, 8 cells; D, 16 cells; and E, 100 cells) and adult worms (G and H) were fixed and then incubated with the affinity-purified antibodies against VHA-5. A-F, in embryos, VHA-5 was significantly observed on a dot-like compartment (A, B, and E, arrowheads) in every cell. Cup-shaped structures (D, arrows) were often detectable instead of dot-like structures (focused on the three fluorescent cells). C and F are phase-contrast images of Band E, respectively. The dark area in each cell in embryos represents the nucleus (E, open arrowhead). G-I, in adult worms, VHA-5 was strongly expressed in an H-shaped excretory cell and was detectable in its cell body (G, arrowheads) and canals (H, arrow). I is a phase-contrast image of H. Scale bars, 20 μm (A, B, D, and E) or 50 μm (G and H). | |||
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Name | F5.large.jpg | ||||
Depict | Expr_pattern | Expr2406 | |||
Anatomy | WBbt:0005812 | ||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2001 | ||||
Article_URL | DOI | id | 10.1074/jbc.M101652200 | ||
Journal_URL | TheJournalofBiologicalChemistry | ||||
Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
Reference | WBPaper00004842 |