WormBase Tree Display for Picture: WBPicture0000008616
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| WBPicture0000008616 | Description | Figure 2. GLD-4 is enriched in the germline and associates with P granules. (A) GLD-4 (caret) is germline-enriched. Protein extracts of embryos (E), adult hermaphrodites of given genotypes probed with a rabbit GLD-4(Ct) antibody. (no GL) glp-1(q224ts) animals without germline; (Pos. cont.) Positive control of a LexA::GLD-4 fusion produced in yeast. (B-F) Extruded germlines (B,C) and embryos (D-F) of wild-type (wt) and gld-4(ef15) adult hermaphrodites stained for chromatin (DAPI), P granules (anti-PGL-1), and GLD-4. (sp) Mature sperm at the proximal end (dashed line). Bar, 50 μm. Insets a-c in B represent a region in the mitotic germline as an optical section generated with an Apotome (Zeiss) microscope. (Inset a) DAPI. (Inset b) PGL-1. (Inset c) GLD-4 staining. (D) Two-cell embryo. (E) Approximately 100-cell embryo. (Arrowheads) Germ cell precursors Z2 and Z3. (F) Epitope-blocked anti-GLD-4(Ct) antibodies (F'') fail to recognize P granules (F') in a dividing two-cell wild-type embryo. | |||
|---|---|---|---|---|---|
| Name | F2.large.jpg | ||||
| Depict | Expr_pattern | Expr8623 | |||
| Anatomy | WBbt:0004575 | ||||
| WBbt:0004576 | |||||
| WBbt:0005784 | |||||
| Cellular_component | GO:0005737 | ||||
| GO:0043186 | |||||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2009 | ||||
| Article_URL | DOI | id | 10.1101/gad.494009 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00033036 |
