WormBase Tree Display for RNAi: WBRNAi00088028
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| WBRNAi00088028 | Homol | Homol_homol | W10G6:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | AGCTTTGGAAGAGTTTACCAAGTCAGGCACAAGGAGACCAAAAAAATCTACGCCATGAAAATTCTGTCCAAGGAACATATCAGAAAAAAGAACGAGGTGAAACACGTCATGGCAGAAAGAAATGTGCTCATTAACAACTTTAAGCACCCTTTCCTTGTTTCACTTCACTTTTCGTTCCAAAATAAGGAGAAGCTGTATTTTGTGCTCGATCACTTGAACGGAGGAGAGCTTTTCTCTCACCTCCAACGCGAGAAACATTTTTCCGAGTCGAGAAGTCGTTTTTACGCGGCCGAGATTGCATGTGCTCTGGGTTATCTTCACGAGAAAAACATTATCTACAGAGATTTGAAGCCTGAAAACCTTCTGTTGGACGATAAAGGTTACTTGGTGCTCACCGACTTTGGGCTCTGTAAAGAAGATATGCAAGGAAGCAAGACAACTTCCACATTTTGTGGGACGCCGGAGTACTTGGCCCCGGAAATTATTCTGAAAAAGCCTTACGACAAGACTGTTGACTGGTGGTGTTTGGGATCGGTTCTCTATGAAATGATTTTTGGATTGCCTCCATTCTACTCAAAAGATCATAACGAGATGTACGACAAGATCATCAACCAGCCACTTCGTCTCAAGCACAACATCTCTGTGCCATGCAGTGAGCTCATCACCGGGCTCTTGCAAAAAG | |||
| Experiment | Laboratory | HT | |||
| Date | 14 May 2010 00:00:00 | ||||
| Genotype | daf-16(mgDf50); daf-16d/f::gfp | ||||
| Treatment | The daf-16(mgDf50) mutants carrying low copies of daf-16d/f::gfp transgene were maintained at 15 degrees Celsius on RNAi plates for two generations. The plates were then transferred to 20 degrees Celsius for 18 hours. About 15-20 L4 worms were then mounted on slides and visualized by fluorescence microscopy. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | W10G6.2a | Inferred_automatically | RNAi_primary | |
| W10G6.2b | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00004789 | Inferred_automatically | RNAi_primary | ||
| Transcript | W10G6.2b.1 | Inferred_automatically | RNAi_primary | ||
| W10G6.2a.1 | Inferred_automatically | RNAi_primary | |||
| W10G6.2a.2 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000518024 | ||||
| Reference | WBPaper00036474 | ||||
| Phenotype_not_observed | WBPhenotype:0000679 | Remark | sgk-1(RNAi) did not result in a significant change in nuclear localization of the DAF-16D/F::GFP product from the daf-16d/f::gfp transgene. | ||
| Remark | sgk-1(RNAi) | ||||
| Method | RNAi |
