WormBase Tree Display for RNAi: WBRNAi00088914
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| WBRNAi00088914 | Homol | Homol_homol | B0303:RNAi | |||
|---|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_B0303.9 | ||||
| Experiment | Laboratory | ALF | ||||
| Date | 21 Mar 2011 00:00:00 | |||||
| Genotype | daf-2(e1371); evIs82b (unc-129::GFP) | |||||
| Treatment | RNAi treatment was performed by feeding animals double-stranded RNA-producing bacteria as described previously except that we used a 4x concentrated RNAi culture to spot plates. After drying, 50-60 eggs isolated by hypochlorite treatment were added to the RNAi plates and kept at 20 degrees Celsius until the worms reached the L4 stage before being transferred to 25 degrees Celsius. Animals were kept at 25 degrees Celsius until Day 10 of adulthood, at which point they were observed for motor neuron morphology. | |||||
| Temperature | 25 | |||||
| Delivered_by | Bacterial_feeding | |||||
| Inhibits | Predicted_gene | B0303.9 | Inferred_automatically | RNAi_primary | ||
| Gene | WBGene00015130 | Inferred_automatically | RNAi_primary | |||
| Transcript | B0303.9.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | |||||
| Reference | WBPaper00038430 | |||||
| Phenotype_not_observed | WBPhenotype:0001331 | Remark | DB and DA motor neurons of animals treated with RNAi appeared to be intact, as determined by unc-129::GFP | |||
| EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
| Remark | B0303.9 (vps-33.1) RNAi | |||||
| Method | RNAi |
