WormBase Tree Display for Strain: WBStrain00051653
expand all nodes | collapse all nodes | view schema
WBStrain00051653 | Status | Live | |
---|---|---|---|
Genotype | unc-119(ox819) F53A2.9(oxTi1127) III. | ||
Public_name | EG9881 | ||
Contains (2) | |||
Properties | Mutagen | mini-Mos+CRISPR_Cas9 | |
CGC_received | 11 Aug 2021 00:00:00 | ||
Location | CGC | ||
Made_by | WBPerson22962 | ||
Remark | oxTi1127 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Inserted into F53A2.9. High Cas9 activity. Integrated Cas9 transgene linked to unc-119(ox819). Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https: | ||
Species | Caenorhabditis elegans |