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WormBase Tree Display for Strain: WBStrain00052772

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Name Class

WBStrain00052772StatusLive
Genotypevit-3(hq485[vit-3::mCherry]) vit-2(crg9070[vit-2::gfp]) X.
Public_nameMQD2775
ContainsGeneWBGene00006926
WBGene00006927
VariationWBVar02158570
PropertiesOutcrossedx6
MutagenCRISPR_Cas9
CGC_received23 Mar 2023 00:00:00
LocationMQD
CGC
Made_byWBPerson30938
Remark[2023-04-25T18:47:30.94Z WBPerson51134] New Strain: submitted by WBPerson143 on 2023-01-29_19:07:36 via the Allele submission form. Genotype vit-2(crg9070[vit-2::gfp]) vit-3(hq485[vit-3::mCherry]) XCurator_confirmedWBPerson51134
mCherry knocked into C terminal of vit-3 by CRISPR/Cas9 in the background of parental strain BCN9071 vit-2(crg9070[vit-2::gfp]) X. This resulting double-labelled strain was crossed six times with N2 to remove potential off-target mutations. mCherry and GFP are co-localized in the intestine, body cavity, oocyte, and embryo in adult hermaphrodites. Reference: Zhai C, et al. Aging cell, 21(11), e13719. https:
SpeciesCaenorhabditis elegans