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WormBase Tree Display for Transgene: WBTransgene00015436

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Name Class

WBTransgene00015436Public_nameWBPaper00041200Ex2
Summary[nhr-239::gfp]
SynonymExpr10053_Ex
ConstructionConstructWBCnstr00015025
Construction_summaryThe nhr-239::gfp plasmid, pNHR239GFP1, was constructed by amplifying a 2.8 kb region from wild-type C. elegans genomic DNA using the following oligonucleotides: OY54IL TTTTCAGATTCTAGGCCGTCAOY5431X CACCCGGGCAACCTTGTGCATTTTGCAA and ligating the product to pPD95.79 using XbaI and XmaI. The resulting plasmid fused 2.1 kb of 5' flanking DNA, which includes the last intron and exon of the adjacent upstream feh-1 gene, and the first three predicted exons of the nhr-239 gene to the coding sequence for GFP.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr10053
ReferenceWBPaper00041200
SpeciesCaenorhabditis elegans