WormBase Tree Display for Transgene: WBTransgene00015870
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| WBTransgene00015870 | Public_name | mdEx990 | |
|---|---|---|---|
| Summary | [FRM394(Punc-17::NYFP::UNC-17(+)); FRM401(Psup-1::SUP-1(+)::CCFP; pBX; pBS] | ||
| Construction | Construct | WBCnstr00015444 | |
| Coinjection_other | pBX | ||
| pBS | |||
| Construction_summary | The pET11a-link-NGFP and pMRBAD-link-CGFP clones were generously provided by the Regan lab (Magliery et al. 2005). The N-terminal portion of GFP (NGFP) was amplified from pET11a-link-NGFP, and cloned into the Fire expression vector pPD95.77. The unc-17 coding sequence from cDNA RM#51p was cloned downstream of the NGFP sequence to yield plasmid RM#935p. The NGFP was converted to NYFP using a fragment amplified from a YFP-containing clone as the primer in a QuikChange reaction. The C-terminal portion of GFP (CGFP) was amplified from pMRBAD-link-CGFP, and cloned into the Fire expression vector pPD95.77. The sup-1 coding sequence from yk1353e09 (without the termination codon) was cloned upstream of the CGFP sequence, with an 8-amino acid linker between SUP-1 and CGFP (RM#934p). The CGFP was then converted to CCFP using QuikChange. | ||
| Genetic_information | Extrachromosomal | ||
| Associated_with | Strain | WBStrain00045167 | |
| Reference | WBPaper00041593 | ||
| Species | Caenorhabditis elegans |
