WormBase Tree Display for Transgene: WBTransgene00018475
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| WBTransgene00018475 | Public_name | WBPaper00042412Ex1 | |
|---|---|---|---|
| Summary | [pan-1prom::GFP; pan-1FL::GFP; rol-6] | ||
| Synonym | Expr11020_Ex | ||
| Construction | Construct | WBCnstr00017859 | |
| Coinjection | WBCnstr00004720 | ||
| Construction_summary | Transcriptional and translational fusions. The pan-1prom::GFP transcriptional fusion was generated by amplifying 3 kb of genomic sequence 5' of the ATG start with hi-fidelity Phusion polymerase (New England Biolabs). The PCR product was cloned into SphI and BamHI sites of pPD95.67 (L2459, Addgene). For the full-length translational GFP fusion, the entire genomic coding sequence of pan-1 consisting of 3kbp of 5' sequence and the coding sequence to the stop codon was PCR amplified and cloned into the SphI and AgeI sites of pPD95.67 in frame with GFP coding sequences. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr11020 | |
| Reference | WBPaper00042412 | ||
| Species | Caenorhabditis elegans |
