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WormBase Tree Display for Variation: WBVar00000104

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Name Class

WBVar00000104EvidencePaper_evidenceWBPaper00005370
NamePublic_nameag1
Other_nameCE31210:p.Gly212Arg
R03G5.2.1:c.634G>A
HGVSgCHROMOSOME_X:g.7818873G>A
Sequence_detailsSMapS_parentSequenceR03G5
Flanking_sequencestagtgtttcgatttacagactgtacaagccgatgcaaaccttacatgccaccagagagaa
Mapping_targetR03G5
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00005370
Person_evidenceWBPerson2292
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00000258
WBStrain00055934
LaboratoryAU
JN
StatusLive
AffectsGeneWBGene00004758
TranscriptR03G5.2.1 (12)
InteractorWBInteraction000503571
WBInteraction000517420
IsolationMutagenEMSPaper_evidenceWBPaper00005370
GeneticsInterpolated_map_positionX-1.13799
DescriptionPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000142Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkUpregulation of pnlp-29::GFP after infection with D. coniospora or after wounding was markedly decreased from that observed for wild type animals.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were infected with D. coniospora at the L4 stage and maintained on OP50 at 20C. Animals were wounded by microinjection needle pricking of the cuticle or by femtosecond laser busts.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
GenotypefrIs7Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0000386Paper_evidenceWBPaper00033433
WBPaper00035318
Curator_confirmedWBPerson2987
RemarkCopper-induced germ cell apoptosis was abolished in sek-1(ag1) mutants (Figure 5B).Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Cobalt did not induce increased germ cell apoptosis in sek-1(ag1) mutants, as opposed to wild type animals (Table 2)Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
WBMol:00003058Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms were exposed to 10 micromolar of copper for 12 hoursPaper_evidenceWBPaper00033433
Curator_confirmedWBPerson2987
Synchronized young adult hermaphrodites were treated in K-medium with 0.01 millimolar Cobalt chloride for 12 hours, and apoptotic cells were scored after AO staining.Paper_evidenceWBPaper00035318
Curator_confirmedWBPerson2987
WBPhenotype:0001012Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
RemarkThis mutant showed no protection from enterotoxigenic Escherichia coli (ETEC) infection when pretreated with Lactobacillus zeae LB1. Authors show that wild type worms had "enhancement in the resistance" to infection by ETEC when worms were first grown on Lactobacillus zeae LB1. This "enhancement in resistance" is not observed in this mutant.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
Phenotype_assayTreatmentAnimals were grown on plates containing K88plus enterotoxigenic Escherichia coli(ETEC) strain JG280 after pretreatment with Lactobacillus zeae LB1.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
WBPhenotype:0001013Paper_evidenceWBPaper00005370
WBPaper00055320
Curator_confirmedWBPerson712
WBPerson557
RemarkWhen exposed to Pseudomonas aeruginosa strain, PA14, under standard assay conditions, 100% esp-2 animals were killed by 31 hours, compared with 0% wild-type animals. Accumulation of GFP-labeled PA14 was detected as early as 6 hours after exposure. Animals were also hypersusceptible to Enterococcus faecalis.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
This mutant is more susceptible to enterotoxigenic Escherichia coli infection compared with N2 wild-type animals.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
Phenotype_assayTreatmentApproximately 25 L4-stage worms were placed on each of three agar plates with PA14 lawns under standard slow killing (SK) assay conditions. Survival was assayed after 30 hours.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Animals grown on plates containing K88plus enterotoxigenic Escherichia coli (ETEC) strain JG280.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
WBPhenotype:0001173Paper_evidenceWBPaper00040855
Curator_confirmedWBPerson557
RemarkAdult males possess an inappropriately surviving linker cell in 49 percent of animals.Paper_evidenceWBPaper00040855
Curator_confirmedWBPerson557
PenetranceIncomplete49 percentPaper_evidenceWBPaper00040855
Curator_confirmedWBPerson557
WBPhenotype:0001351Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkAnimals had diminished levels of p38 MAP kinase activation compared to wild-type N2 worms, when in the presence of PA14, as determined by immunoblot analysis of lysates from mutants probed with anti-phospho-p38 and anti-PMK-1 with anti-beta-tubulin as a loading control.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Phenotype_assayTreatmentSynchronized L4 populations of N2 wild type, esp-2/sek-1(ag1), and esp-8/nsy-1(ag3) worms were transferred to PA14 plates in parallel, and lysates were prepared for immunoblotting after 6 hours when the worms were predominantly young adults.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0001661Paper_evidenceWBPaper00044621
Curator_confirmedWBPerson6538
RemarkFig 5. Loss of AWC asymmetryPaper_evidenceWBPaper00044621
Curator_confirmedWBPerson6538
PenetranceHighPaper_evidenceWBPaper00044621
Curator_confirmedWBPerson6538
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00044621
Curator_confirmedWBPerson6538
EQ_annotationsAnatomy_termWBbt:0005672PATO:0000460Paper_evidenceWBPaper00044621
Curator_confirmedWBPerson6538
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkLongevity of animals was equivalent to wild-type worms.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
Phenotype_assayTreatment25 L4 animals were placed on each of three plates that contained 5-fluoro-2-deoxyuridine and had been seeded with E. coli OP50.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000650Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000659Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0000676Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
RemarkWhen grown on E. coli OP50 animals develop at a normal rate and reach adulthood concomitant with N2 wild-type worms.Paper_evidenceWBPaper00005370
Curator_confirmedWBPerson712
WBPhenotype:0001725Paper_evidenceWBPaper00032031
Curator_confirmedWBPerson712
RemarkOsmotic stress triggered upregulation of nlp-29 was not affected, based on the fluorescent ratio of control to experiment animals.Paper_evidenceWBPaper00032031
Curator_confirmedWBPerson712
Phenotype_assayTreatmentOsmotic stress was done in liquid by incubating young adult worms in 300 mM NaCl for 6 h, with analysis occuring 24 hours later.Paper_evidenceWBPaper00032031
Curator_confirmedWBPerson712
GenotypefrIs7Paper_evidenceWBPaper00032031
Curator_confirmedWBPerson712
ReferenceWBPaper00040855
WBPaper00033433
WBPaper00032031
WBPaper00005370
WBPaper00031666
WBPaper00035318
WBPaper00044621
WBPaper00055320
WBPaper00066032
MethodSubstitution_allele